10 A Piece of the PuzzleUnlock a piece of Subject 16's video.
10 Art ConnoisseurBuy a Painting from Florence and Venice.
10 Assassin For HireComplete your first assassination mission for Lorenzo Il Magnifico.
10 Handy ManUpgrade a building in the Stronghold.
10 High DivePerform a Leap of Faith from the Top of Florence's Giotto's Campanile.
10 I like the viewSynchronize 10 View Points.
10 KleptomaniacPickpocket 1000 Florins.
10 Lightning StrikeSprint for 100 meters.
10 Macho ManDefend a woman's honor.
10 MailmanIntercept a Borgia Courrier.
10 Man of the PeopleToss more than 300 florins on the ground.
10 Messer SandmanStun 4 guards at once by throwing sand in their face.
10 Perfect HarmonyTint your clothes with those colors: Wetland Ebony and Wetland Ivory.
10 Red Light AddictSpend 5000 florins on Courtesans.
10 Show your ColorsWear the Auditore cape in each city.
10 Steal HomeWin a race against thieves!
10 Street CleanerHide 5 dead bodies in a Bale of Hay.
10 SweeperSweep 5 guards at once by using a Long Weapon.
10 Tip of the IcebergUse your Eagle Vision to scan a Glyph in the environment.
10 Victory lies in preparationGet all Hidden Blades, Item Pouches and Armor upgrades for Ezio.
100 An Old Friend ReturnsEscape the hideout.
20 Arrivederci AbstergoBreak out of Abstergo.
20 Choir BoyDiscover the Assassin's Tomb inside Santa Maria del Fiore (The Duomo).
20 DoctorPerform an Air Assassination on a Poisoned NPC.
20 Hallowed be thy nameDiscover the Assassin's Tomb inside the Basilica di San Marco.
20 I can see your house from here!Discover the Assassin's Tomb inside the Torre Grossa.
20 No-hitterKill 10 enemies while remaining in conflict without being hit.
20 Prison EscapeDiscover the Assassin's Tomb inside the Rocca di Ravaldino fortress.
20 The Birth of an AssassinBe reborn as Ezio Auditore Da Firenze.
20 UndertakerDiscover the Assassin's Tomb inside the catacombs under Santa Maria Novella.
20 Venetian GladiatorDiscover the Assassin's Tomb inside Santa Maria della Visitazione.
20 Vitruvian ManUnlock all 20 pieces of Subject 16's video.
20 Welcome to the Animus 2.0Enter the Animus 2.0.
30 An Unexpected JourneyComplete DNA Sequence 6.
30 Bianca's ManComplete DNA Sequence 10.
30 Bleeding EffectComplete training and reenter the Animus.
30 Bloody SundayComplete DNA Sequence 4.
30 Exit the SonComplete DNA Sequence 3.
30 In Memory of PetruccioCollect all the Feathers.
30 MasqueradeComplete DNA Sequence 9.
30 Podestà of MonteriggioniReach 80% of your stronghold's total value.
30 The ConspiratorsComplete DNA Sequence 5.
30 The Impenetrable PalazzoComplete DNA Sequence 8.
30 The Merchant of VeniceComplete DNA Sequence 7.
30 The Pain of BetrayalComplete DNA Sequence 1.
30 The ProphetComplete DNA Sequence 11.
30 The VaultComplete DNA Sequence 14.
30 VengeanceComplete DNA Sequence 2.
5 Fly SwatterKick a Guard while using the Flying Machine.
5 Myth MakerFind the 8 statuettes in Monteriggioni.