The following bonus characters become available when you have beaten the game and then entered the corresponding code at the 'Cheat' menu.

Unlock Moon Knight:
Enter the code CRESTWARRIOR

Unlock Rhino:
Enter the code MASSIVEHORN

Unlock Venom:
Enter the code IEATYOURBRAINS

This feature becomes available when you have collected 1,000 Spider Tokens, when you complete the task ALL the Spider Tokens will appear as red dots on your mini map.

When you fight Wolverine he will ask you a series of questions that need be answered correctly, below are the answers to that quiz.

Question 1:
Answer: Richard and Mary

Question 2:
Answer: Ned Leeds

Question 3:
Answer: Battleworld

Question 4:
Answer: Daredevil

Question 5:
Answer: A Mutant

Question 6:
Answer: May Parker

If you complete the following tasks you will be able to get an assist from the corresponding character.

Big Wheel:
After beating Tinkerer keep selecting the 'bad' choice when asked by computer.

Black Cat:
Select to save Black Cat when you fight her (pick the 1st choice when asked by Smythe)

Hobgoblin:
Before fighting Kraven keep selecting the 'bad' choice (the 2nd/3rd choice).

Jessica Drew:
Before you fight with Jessica Drew Select to help S.H.I.E.L.D crew from the symbiote.

Luke Cage:
Select the 'bad' choice (2nd choice) when you asked by a man who says he can divert the train containing symbiote.

Shocker:
Choose to free Shocker when you have defeated him.

Solidarity:
When you have defeated Shocker take him to jail.

Storm:
When you have defeated Tinkerer keep selecting the 'good' answer when asked by computer.

Random Assist:
When you beat any level or buy the ultra rare power ups you will be able to get a random assist from the following characters - Mysterio, Night Crawler, Wolverine, Galactus, Hypno Hustler Ctylok.

The following bonus costumes become available options when you complete the indicated task during the game.

Unlock Cosmic Spider-Man:
Complete Act 1

Unlock Spider-Carnage:
Defeat 500 enemies

Unlock Ben Reily:
Complete 25 City events.

Unlock Spider-Man 2099:
Complete 50 City events.