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In the castle around chapter 3-2, before Ashley gets captured, there will be a room with four paintings on the wall. And it will say the sacrifice of six lives is needed to open the door. Simply press 1 3 2 4, and the door should open. This puzzle is simple,yet can be annoying.
Hope it helps.
More tricks, tips and cheats for this game are right here - Resident Evil 4 cheats
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