Unlock Kengo Mizumachi:
Beat the game with Eyeshield 21: Devilbats Devildays in the DS

Unlock Shun Kakei:
Beat the game with Eyeshield 21: Devilbats Devildays in the DS

Unlock Kyoshin Poseidon Characters and Uniforms:
Beat the game with Eyeshield 21: Max Devilpower and Eyeshiled 21: Devilbats Devildays in your DS at the same time. Hold L when choosing your team which must be an edit team with Shun and Kengo in it.

Unlock NASA Aliens Team:
When Story mode ends the defeat the NASA Aliens team which challenge you to unkock them.